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Updated: Jun 26, 2026

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
Published on: June 16, 2020
The influence of chromatic components on extended depth of field imaging
1Physical Chemistry Department, Science Faculty, University of Cadiz, Pol. Río San Pedro s/n, Puerto Real, Cádiz, Spain. javier.navas@uca.es
Abstract:
The analysis of variance is one of the most commonly used algorithms for detecting focalized zones in digital images and is an entry point to extended focalization techniques beyond those established by optical laws. This article analyses the dependence of the red, green, and blue (RGB) and lightness, hue, and saturation (LHS) components when used as the basis for applying an algorithm to obtain images with extended depth of field. Also, an algorithm developed by the authors is described and the dependence of the final result is shown according to the chromatic components used as the variables. Finally, a methodology is defined based on the study of second variance in relation to the number of images and pixels of the chromatic coordinates to decide which to use as the basis for the calculation.
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