Related Experiment Video
Updated: Jun 26, 2026
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
In vitro elution of moxifloxacin from cancellous bone allografts
Kyriaki Kanellakopoulou1, Theophilos Sahinides, Thomas Tsaganos
1Fourth Department of Internal Medicine, ATTIKON University Hospital, 1 Rimini Str, Athens 124 62, Greece.
Abstract:
The characteristics of cancellous bone allografts as carriers of moxifloxacin are described. Particles of cancellous bone were compressed into a wire-mesh cylinder and impregnated into a solution of moxifloxacin for different time periods. Five replicas were impregnated for 1 h; another five for 24 h; and another five for 48 h. Impregnated allografts were then transferred into vials containing 5 ml of Mueller-Hinton broth and incubated at 37 degrees C. Broth was replaced daily. Concentrations of moxifloxacin in broth were determined after analysis by an high performance liquid chromatography system. Moxifloxacin was eluted at very high concentrations within the first days. Concentrations remained above 100 microg/ml until day 8 and above 40 microg/ml until day 20. It is concluded that cancellous bone allografts may allow the adequate in vitro elution of moxifloxacin. The latter results support their application in experimental models of osteomyelitis.
Insights
Cancellous bone allografts effectively carry moxifloxacin, releasing high concentrations for extended periods. This study supports their use in experimental osteomyelitis models.
Area of Science:
- Biomaterials Science
- Pharmacology
- Orthopedic Surgery
Background:
- Cancellous bone allografts are potential drug delivery vehicles.
- Osteomyelitis treatment requires effective antibiotic delivery to bone tissue.
Purpose of the Study:
- To characterize the elution profile of moxifloxacin from cancellous bone allografts.
- To evaluate the suitability of these allografts as carriers for treating osteomyelitis.
Main Methods:
- Cancellous bone particles were formed into cylinders and impregnated with moxifloxacin for varying durations (1, 24, 48 hours).
- Elution of moxifloxacin was measured daily in Mueller-Hinton broth using high-performance liquid chromatography (HPLC).
Main Results:
- Moxifloxacin eluted at high concentrations within the initial days.
- Concentrations remained above 100 µg/ml for 8 days and above 40 µg/ml for 20 days.
- Elution kinetics were dependent on impregnation time.
Conclusions:
- Cancellous bone allografts demonstrate adequate in vitro elution of moxifloxacin.
- These findings support the potential application of moxifloxacin-loaded allografts in experimental osteomyelitis models.
