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Updated: Jun 26, 2026
High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
[Studies on chemical constituents of Ranunculus japonicus]
Yong-Feng Liang1, Zuo-Tao Chen, Li-Hong Liu
1College of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, China. qylyf338@yahoo.com.cn
Objective:
To isolate and determine the chemical constituents of Ranunculus japonicus in Liupan Mountain, Ningxia province, China.
Method:
The herb was extracted with ethanol by ultrasonic bath. The extractives were divided to petroleum ether, ethyl acetate, n-butanol parts. The first two parts were separated and purified with silica gel and Sephadex LH -20 column chromatography. The structures of the separated compounds were idnetefied by physical and chemical properties and spectral analysis.
Result:
Nine compounds were isolated and identified as follows: scoparone (1), tricin (2), protocatechuic acid (3), luteolin (4), anemonin (5), scopoletin (6), 5-hydroxy-6, 7-dimethoxyflavone (7), ternatolide (8), 5-hydroxy-7, 8-dimethoxy-flavone (9).
Conclusion:
Compounds 1-9 were isolated from Ranunculus japonicus for the first time.
