A mineral trioxide aggregate sealer stimulated mineralization

Area of Science:

• Biomaterials Science
• Dental Materials Science
• Tissue Engineering

Background:

• Dental pulp capping and root canal sealers are crucial for endodontic treatment success.
• Biocompatibility of root canal sealers is paramount to prevent adverse tissue reactions.
• Portland cement-based materials have emerged as promising alternatives in endodontics.

Purpose of the Study:

• To evaluate the subcutaneous tissue response of Wistar rats to Endo-CPM-Sealer compared to Sealapex and Angelus MTA.
• To assess the biocompatibility and mineralization potential of Endo-CPM-Sealer.
• To compare the healing and inflammatory responses induced by different root canal sealers.

Main Methods:

• Polyethylene and dentin tubes filled with Endo-CPM-Sealer, Sealapex, or Angelus MTA were implanted into rat dorsal connective tissue.
• Implantations were evaluated at 7, 15, 30, 60, and 90 days.
• Histological analysis included hematoxylin and eosin staining, Von Kossa staining for mineralization, and polarized light microscopy for birefringent materials.

Main Results:

• All tested materials elicited mild to moderate inflammatory reactions initially, which subsided over time.
• Endo-CPM-Sealer and Angelus MTA showed responses similar to the control by day 30.
• Sealapex exhibited a similar tissue response by day 60.
• Mineralization and birefringent granulation tissue were observed with all tested sealers.

Conclusions:

• Endo-CPM-Sealer exhibits good biocompatibility, comparable to Sealapex and Angelus MTA.
• Endo-CPM-Sealer actively stimulates mineralization in the surrounding subcutaneous tissue.
• The findings support the potential of Endo-CPM-Sealer as a viable root canal sealing material.