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Updated: Jun 26, 2026
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High-throughput Screening for Protein-based Inheritance in S. cerevisiae
Published on: August 8, 2017
Increased -1 ribosomal frameshifting efficiency by yeast prion-like phenotype [PSI+]
Hee Jung Park1, So Jung Park, Doo-Byoung Oh
1Department of Chemistry, School of Natural Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
FEBS Letters
|January 27, 2009
Summary
Programmed -1 ribosomal frameshifting (-1RF) efficiency increases dramatically in [PSI+] cells, which modify translation termination. Curing [PSI+] abolished these increases, suggesting translation perturbation enhances -1RF.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Programmed -1 ribosomal frameshifting (-1RF) is a translational process regulated by slippery sequences and RNA structures.
- Translational fidelity can be influenced by epigenetic modifiers like [PSI+].
Purpose of the Study:
- To investigate the impact of the [PSI+] factor on -1RF efficiency.
- To determine if translational perturbation in [PSI+] cells affects -1RF frequency.
Main Methods:
- Analysis of -1RF efficiency in yeast strains with and without the [PSI+] factor.
- Comparison of -1RF frequencies at specific slippery sequences.
Main Results:
- A dramatic increase in -1RF efficiency was observed at slippery sequences within [PSI+] cells.
- Abolition of [PSI+] (curing) led to the disappearance of enhanced -1RF efficiencies.
- Enhanced -1RF frequency is linked to the perturbation of the translation process in [PSI+] cells.
Conclusions:
- The [PSI+] factor significantly enhances -1RF efficiency at specific sequences.
- Translational perturbation induced by [PSI+] is a key factor in modulating -1RF frequency.
- -1RF modulation by [PSI+] represents a novel physiological effect of altered translation termination fidelity.
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