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Updated: Jun 26, 2026

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Published on: January 1, 2016
Dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-UV detection as a very
Mohammad Rezaee1, Yadollah Yamini, Shahab Shariati
1Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Abstract:
Dispersive liquid-liquid microextraction (DLLME) coupled with high-performance liquid chromatography (HPLC)-UV detection was applied for the extraction and determination of bisphenol A (BPA) in water samples. An appropriate mixture of acetone (disperser solvent) and chloroform (extraction solvent) was injected rapidly into a water sample containing BPA. After extraction, sedimented phase was analyzed by HPLC-UV. Under the optimum conditions (extractant solvent: 142 microL of chloroform, disperser solvent: 2.0 mL of acetone, and without salt addition), the calibration graph was linear in the range of 0.5-100 microgL(-1) with the detection limit of 0.07 microgL(-1) for BPA. The relative standard deviation (RSD, n=5) for the extraction and determination of 100 microgL(-1) of BPA in the aqueous samples was 6.0%. The results showed that DLLME is a very simple, rapid, sensitive and efficient analytical method for the determination of trace amount of BPA in water samples and suitable results were obtained.
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