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Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Master Transcription Regulators

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Related Experiment Video

Updated: Jun 26, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Transcriptomic profiling identifies a PU.1 regulatory network in macrophages.

Karin Weigelt¹, Monika Lichtinger, Michael Rehli

¹Institute of Human Genetics, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany.

Biochemical and Biophysical Research Communications

|January 27, 2009

Summary

This summary is machine-generated.

This study reveals the PU.1 transcriptional network, identifying novel target genes crucial for macrophage development. Researchers mapped gene expression and PU.1 binding sites to understand its regulatory role in hematopoiesis.

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

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Published on: June 15, 2016

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

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Area of Science:

Molecular Biology
Genomics
Immunology

Background:

PU.1 is a critical transcription factor regulating hematopoiesis and macrophage differentiation.
Previous studies identified PU.1 target genes using chromatin immunoprecipitation.

Purpose of the Study:

To complement existing knowledge by performing transcriptomic analysis of PU.1(-/-) progenitors after PU.1 activity restoration.
To define a multi-level PU.1 regulatory network in macrophages.

Main Methods:

Transcriptomic analysis using Affymetrix exon and genome arrays.
Integrated analysis of genome-wide expression data with public microarray datasets.
Utilized bibliographic gene connections, binding site prediction, and ChIP-Chip data.

Main Results:

Identified novel PU.1 target genes and a comprehensive PU.1 transcriptional network.
Discovered an alternative transcript of the PU.1 target gene Ptpro via exon arrays.
Demonstrated PU.1 binding to an intronic promoter of Ptpro.

Conclusions:

Presents a validated PU.1 transcriptional network with novel target genes.
Provides insights into PU.1's regulatory role in macrophage differentiation and hematopoiesis.