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Updated: Jun 26, 2026

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
Immobilized metal affinity chromatography in the presence of arginine
Ryota Abe1, Motonori Kudou, Yoshikazu Tanaka
1Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 301 FBS-Bldg., 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan.
Abstract:
Arginine hydrochloride (ArgHCl) is a versatile solvent additive, as it suppresses protein aggregation. ArgHCl has been used for protein refolding and to solubilize proteins from loose inclusion bodies. Immobilized metal affinity chromatography (IMAC) is one of the most commonly used technologies for purification of recombinant proteins. Here we have evaluated compatibility of ArgHCl with IMAC purification for his-tag proteins. ArgHCl clearly interfered with protein binding to Ni-columns. Nevertheless, such interference was greatly reduced at ArgHCl concentration below 200 mM, demonstrating that IMAC purification can be done even in the presence of ArgHCl.
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