Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Implementation of an AI System for Generating Clinical Urine Drug Test Sign-Outs.

JAMA network open·2026
Same author

Phenotype-Specific Recalibration of MAVE Data Enables Repurposing of <i>BAP1</i> Functional Assays for Küry-Isidor Syndrome.

medRxiv : the preprint server for health sciences·2026
Same author

International expert consensus recommendations on standardised nomenclature of SSA/Ro (TROVE2/Ro60 and TRIM21/Ro52) autoantibodies in autoimmune diseases.

Annals of the rheumatic diseases·2026
Same author

Genome-wide detection and clinical prioritization of tandem repeat outliers using long-read sequencing.

medRxiv : the preprint server for health sciences·2026
Same author

Reducing the Harm of Urine Drug Screening in Pregnancy.

JAMA network open·2025
Same author

Payment Matters: Understanding Payer Perspectives on Laboratory Stewardship.

The journal of applied laboratory medicine·2025
Same journal

From Pooled Cohorts to PREVENT: A Perspective for Clinical Laboratorians.

Clinical chemistry·2026
Same journal

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
12:30

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Published on: May 19, 2017

Reducing routine ionized calcium measurement.

Geoffrey S Baird1, Petrie M Rainey, Mark Wener

  • 1Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195-7110, USA. gbaird@u.washington.edu

Clinical Chemistry
|January 27, 2009
PubMed
Summary
This summary is machine-generated.

Implementing a reflexive calcium testing strategy significantly reduced ionized calcium (iCa) testing and intravenous calcium supplementation. This approach, targeting patients with low total calcium (tCa), did not increase adverse clinical outcomes.

More Related Videos

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
10:24

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Published on: October 28, 2014

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

Related Experiment Videos

Last Updated: Jun 26, 2026

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
12:30

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Published on: May 19, 2017

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
10:24

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Published on: October 28, 2014

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

Area of Science:

  • Clinical Chemistry
  • Hospital Laboratory Medicine
  • Critical Care Medicine

Background:

  • Ionized calcium (iCa) testing is frequently performed in hospitalized patients, often revealing hypocalcemia.
  • The routine practice of iCa testing and subsequent calcium supplementation warrants critical evaluation.

Purpose of the Study:

  • To assess the impact of a reflexive calcium testing strategy on iCa testing frequency and calcium supplementation.
  • To determine if this strategy affects clinical outcomes associated with hypocalcemia.

Main Methods:

  • Retrieved data for 58,040 ionized calcium (iCa) tests and calcium supplementation records.
  • Evaluated testing frequency, hypocalcemia rates, and effects of intravenous (IV) and oral calcium therapy.
  • Implemented and analyzed a reflexive strategy based on total calcium (tCa) levels.

Main Results:

  • Serial and daily iCa testing constituted a significant portion of all tests ordered.
  • Half of tested patients exhibited hypocalcemia; IV calcium therapy showed a small effect compared to spontaneous iCa increases.
  • A low total calcium (tCa) threshold (<2.00 mmol/L) effectively identified most patients with low iCa (<1.0 mmol/L).
  • The reflexive strategy reduced iCa testing by 72%-76% and IV calcium gluconate therapy by 45%-81%.

Conclusions:

  • A reflexive calcium testing strategy, limiting iCa tests to patients with low tCa, significantly decreased testing and IV calcium supplementation.
  • This strategy did not lead to an increase in adverse clinical outcomes potentially related to hypocalcemia.