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SPARC-null mice exhibit lower intraocular pressures.

Ramez I Haddadin1, Dong-Jin Oh, Min Hyung Kang

  • 1Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA.

Investigative Ophthalmology & Visual Science
|January 27, 2009
PubMed
Summary

SPARC-null mice exhibit lower intraocular pressure (IOP) due to enhanced aqueous outflow. This study investigated SPARC

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Physiology

Background:

  • SPARC (secreted protein acidic and rich in cysteine) is a matricellular protein found in tissues undergoing remodeling.
  • It is notably present in the eye's trabecular meshwork and ciliary body, structures critical for regulating intraocular pressure (IOP).

Purpose of the Study:

  • To investigate the role of SPARC in the regulation of intraocular pressure (IOP).
  • To compare IOP in SPARC-null mice with wild-type (WT) and heterozygous counterparts.

Main Methods:

  • Measurement of diurnal and nocturnal IOP in C57Bl/6x129SvJ WT, SPARC-null, and heterozygous mice.
  • Assessment of aqueous humor turnover using fluorophotometry.
  • Measurement of central corneal thickness (CCT) via histology, ultrasound biomicroscopy, and OCT.

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  • Examination of iridocorneal angles using light microscopy.

    • Main Results:

    • SPARC-null mice demonstrated significantly lower daytime IOP compared to WT and heterozygous mice (16.9 vs. 19.9 and 19.3 mm Hg, respectively).
    • SPARC-null mice showed a blunted IOP increase at night.
    • Central corneal thickness and iridocorneal angle morphology were similar between SPARC-null and WT mice.
    • Aqueous humor turnover rates were comparable or higher in SPARC-null mice, suggesting enhanced outflow.

    Conclusions:

    • SPARC deficiency leads to reduced intraocular pressure in mice.
    • The findings suggest that SPARC influences IOP regulation potentially through enhanced aqueous humor outflow resistance.