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Related Experiment Videos

Mast cell adhesion to fibronectin.

J Dastych1, J J Costa, H L Thompson

  • 1Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Immunology
|August 1, 1991
PubMed
Summary
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Mast cells, including MCP-5 and bone marrow-derived cultured mast cells (BMCMC), bind to fibronectin, an extracellular matrix protein. This adhesion is mediated by the RGDS cell attachment site, suggesting roles in tissue localization, wound healing, and fibrosis.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Mast cells are immune cells involved in allergic reactions and inflammation.
  • Extracellular matrix proteins, like fibronectin, play crucial roles in cell adhesion and tissue structure.
  • Previous research indicated mast cell interactions with laminin, another matrix protein.

Purpose of the Study:

  • To investigate the interaction between mast cells and fibronectin.
  • To determine the specific binding sites and mechanisms involved in mast cell adhesion to fibronectin.
  • To elucidate the implications of mast cell-fibronectin interactions in physiological processes.

Main Methods:

  • Utilized the MCP-5 murine mast cell line and primary bone marrow-derived cultured mast cells (BMCMC).

Related Experiment Videos

  • Assessed mast cell adhesion to fibronectin using dose-response experiments.
  • Investigated the role of the RGDS (arginine-glycine-aspartic acid) cell attachment site using fibronectin fragments and peptides.

    • Main Results:

    • Both MCP-5 and BMCMC demonstrated dose-dependent binding to fibronectin, with optimal adhesion at 20 µg/ml.
    • Activated BMCMC and spontaneously adhering MCP-5 cells showed fibronectin binding.
    • A 120,000 MW fibronectin fragment containing the RGDS site promoted mast cell attachment, and RGDS peptide inhibited this adhesion.

    Conclusions:

    • Mast cells adhere to fibronectin via the RGDS cell attachment site.
    • These findings expand the understanding of mast cell interactions with the extracellular matrix beyond laminin.
    • The interaction suggests potential roles for mast cells in tissue localization, wound healing, and fibrotic processes.