Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid serodiagnostic test for pulmonary tuberculosis.

V V Radhakrishnan1, A Mathai, B Sunderam

  • 1Department of Pathology, Institute for Medical Sciences and Technology, Trivandrum, India.

Indian Journal of Experimental Biology
|May 1, 1991
PubMed
Summary

Counter-current immunoelectrophoresis (CIE) detected specific mycobacterial antibodies in immune complexes (ICs) from pulmonary tuberculosis (PT) patients. This simple test is valuable for diagnosing PT, especially when sputum tests are negative.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial neurogastrointestinal encephalopathy (MNGIE) in a South Indian family with two affected siblings.

Neurology India·2018
Same author

Angiographic predictors of success in antegrade approach of Chronic Total Occlusion interventions in a South Indian population in the contemporary era.

Indian heart journal·2018
Same author

Aortic pulse wave velocity and its relationship with complexity of coronary artery disease based on SYNTAX score.

Heart Asia·2016
Same author

A study of ECG pattern, cardiac structural abnormalities and familial tendency in patients with early repolarisation syndrome in South India.

Heart Asia·2016
Same author

Combination of peripheral laser photocoagulation with intravitreal bevacizumab in naïve eyes with macular edema secondary to CRVO: prospective randomized study.

Eye (London, England)·2016
Same author

Tumefactive demyelinating lesions: a clinicopathological correlative study.

Indian journal of pathology & microbiology·2013

Area of Science:

  • Immunology
  • Medical Diagnostics
  • Microbiology

Background:

  • Immune complexes (ICs) play a role in various diseases.
  • Diagnosing pulmonary tuberculosis (PT) can be challenging, particularly with negative sputum bacteriology.

Purpose of the Study:

  • To investigate the presence of specific mycobacterial antibodies in ICs of PT patients.
  • To evaluate counter-current immunoelectrophoresis (CIE) as a potential serodiagnostic tool for PT.

Main Methods:

  • Immune complexes were isolated from patient and control sera using polyethylene glycol (PEG) precipitation.
  • Specific mycobacterial antibodies within ICs were detected using counter-current immunoelectrophoresis (CIE) against a purified mycobacterial antigen.

Main Results:

Related Experiment Videos

  • Specific mycobacterial antibodies were found in the ICs of 70% of PT patients.
  • No specific antibodies were detected in ICs from non-tuberculous pulmonary disease patients or healthy controls.

Conclusions:

  • CIE is a sensitive and rapid method for detecting specific mycobacterial antibodies in PT patients' ICs.
  • CIE offers advantages over ELISA and is suitable for resource-limited laboratories for PT diagnosis.