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Updated: Jun 26, 2026

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
Published on: June 11, 2011
[HIV/HCV coinfection at an university hospital in Recife, Brazil]
Flávia Helena Pontes de Carvalho1, Maria Rosângela Cunha Duarte Coêlho, Tatiana de Aguiar Santos Vilella
1Setor de Virologia, Laboratório de Imunopatologia Keizo-Asam, Universidade Federal de Pernambuco, Recife, PE, Brasil.
Objective:
To estimate the prevalence of hepatitis C virus (HCV) infection and risks factors associated with coinfection in HIV-positive individuals.
Methods:
A cross-sectional descriptive study was conducted with 343 HIV patients attended at a university hospital in Recife, Northeastern Brazil, from March to December 2003. A standardized questionnaire about risk factors was administered. Serum samples were analyzed for anti-HCV antibodies using enzyme-linked immunosorbent assay (ELISA), HCV-RNA using reverse transcription-polymerase chain reaction (RT-PCR), and genotyping using the ABI 377 (PE Biosystems). Univariate and multivariate analyses and multiple logistic regression were performed.
Results:
HCV prevalence was 4.1% (14/343) using ELISA and 3.2% (11/343) using RT-PCR. The most common genotypes were 1b (45%), 3 (33%) and 1a (22%). Co-infection was higher among those aged 30 to 39 years, and predominantly in males (64.3 %). In the multiple logistic regression, the variable blood transfusion was the single remaining risk factor for HCV (OR=4.28; 95% CI 1.44;12.73).
Conclusions:
The prevalence of HIV/HCV coinfection was low. Blood transfusion was a risk factor and HCV genotype 1b was the most frequently found.
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