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The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques
Published on: July 3, 2014
CD38 in bovine lung: A multicatalytic NADase
Valeria Polzonetti1, Stefania Pucciarelli, Alberto Vita
1Dipartimento di Scienze Morfologiche e Biochimiche Comparate, Università di Camerino, Italy. valeria.polzonetti@unicam.it
The Journal of Membrane Biology
|January 27, 2009
Summary
This study reveals CD38 as a novel enzyme with multiple functions, including NADase and cyclase activities. It plays a crucial role in regulating cellular energy and signal transduction by managing NAD(+) and calcium-mobilizing agents.
Area of Science:
- Biochemistry
- Enzymology
- Molecular Biology
Background:
- CD38 is an enzyme found in cellular membranes.
- Its precise enzymatic activities and biological roles are not fully understood.
Purpose of the Study:
- To elucidate the kinetics and molecular properties of CD38.
- To characterize its catalytic functions, including NAD(+) metabolism and calcium signaling.
Main Methods:
- Purification of CD38 from bovine lung microsomal membranes.
- Solubilization using Triton X-100.
- Enzymatic assays to determine substrate utilization and product formation.
Main Results:
- CD38 exhibits multicatalytic NAD(+)-glycohydrolase (NADase) activity.
- It produces nicotinamide and either ADPR (NADase) or cADPR (cyclase activity).
- CD38 also hydrolyzes cADPR and catalyzes pyridine base exchange reactions, forming NAD analogues.
Conclusions:
- CD38 is a versatile enzyme involved in NAD(+) metabolism.
- It regulates calcium-mobilizing agents, impacting cellular energy and signal transduction.
- CD38 is a key enzyme in cellular energy homeostasis and signaling pathways.
