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Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
Published on: May 13, 2019
Sequence analysis of the plasmid pGY1 harbored in Salmonella enterica serovar paratyphi A
1Department of Clinical Biochemistry, Guiyang Medical College, Guiyang, People's Republic of China. huanghai828@gmc.edu.cn
Abstract:
The cryptic plasmid pGY1, which is harbored by a clinical isolate of Salmonella enterica serovar Paratyphi A, was identified in a 9-year-old girl with paratyphoid fever in 2005, and its DNA sequence was determined. It is 3592 bp in length and had a G+C content of 43.3%. Three ORFs were predicted that share low similarity with hypothetical proteins in the GenBank database. pGY1 shared 36.6% sequence homology with the cryptic plasmid pIMVS1 from Salmonella typhimurium. Its unique sequence makes it attractive for further study to obtain insight into the evolutionary relationship of this plasmid with other Enterobacteriaceae plasmids.

