Studies on paclitaxel-loaded glyceryl monostearate nanoparticles

Area of Science:

• Nanotechnology
• Pharmaceutical Sciences
• Drug Delivery

Background:

• Paclitaxel is a potent chemotherapeutic agent with limited solubility.
• Solid lipid nanoparticles (SLNs) offer a promising platform for improving drug delivery and bioavailability.
• Glyceryl monostearate (GMS) is a suitable lipid excipient for SLN formulation.

Purpose of the Study:

• To prepare and characterize Paclitaxel-loaded SLNs using Glyceryl monostearate (GMS).
• To evaluate the physicochemical properties and drug release kinetics of the prepared SLNs.
• To assess the anti-proliferative activity of encapsulated Paclitaxel in B16F10 cell lines.

Main Methods:

• Modified Hot homogenization method was employed for SLN preparation.
• Physicochemical characterization included particle size, entrapment efficiency, and zeta potential analysis.
• Transmission Electron Microscopy (TEM) was used for morphological evaluation.
• In vitro drug release studies were conducted at different pH values.
• Chemosensitivity assays were performed on B16F10 cell lines.

Main Results:

• Paclitaxel-loaded SLNs exhibited a mean particle size of 226 nm and high entrapment efficiency (92.43%).
• TEM confirmed the spherical morphology of the SLNs with a zeta potential of -29.4 mV.
• Drug release followed Higuchi diffusion at pH 7.4 and Peppas-Korsenmeyer model at pH 3.5.
• Encapsulation did not impede the anti-proliferative activity of Paclitaxel against B16F10 cells.

Conclusions:

• The modified Hot homogenization method is effective for preparing Paclitaxel-loaded SLNs using GMS.
• The prepared SLNs possess favorable physicochemical properties for drug delivery.
• Paclitaxel-loaded SLNs retain their anti-cancer efficacy, suggesting their potential as a viable drug delivery system.