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Related Experiment Video

Updated: Jun 26, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

CCCTC-binding factor meets poly(ADP-ribose) polymerase-1.

Paola Caiafa¹, Jordanka Zlatanova

¹Department of Cellular Biotechnology and Haematology, II Faculty of Medicine and Surgery, University La Sapienza, Pasteur Institute-Fondazione Cenci Bolognetti, Rome, Italy. caiafa@bce.uniroma1.it

Journal of Cellular Physiology

|January 27, 2009

Summary

The CCCTC-binding factor (CTCF) protein

Area of Science:

Molecular Biology
Epigenetics
Gene Regulation

Background:

CCCTC-binding factor (CTCF) is a multifunctional protein involved in gene regulation.
CTCF interacts with diverse proteins and undergoes post-translational modifications like poly(ADP-ribosyl)ation (PARylation).

Purpose of the Study:

To summarize and critically discuss the role of CTCF PARylation in gene imprinting and ribosomal gene transcription.
To contextualize these findings within the broader activities of PARP, including genome methylation regulation.

Main Methods:

Literature review and critical discussion of existing data.
Analysis of the interplay between CTCF, PARylation, and epigenetic regulation.

Main Results:

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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PARylated CTCF is implicated in gene imprinting.

PARylated CTCF plays a role in controlling ribosomal gene transcription.

Protein PARylation by PARP is crucial for regulating genome methylation patterns.

Conclusions:

CTCF's diverse functions are mediated by protein interactions and post-translational modifications.
PARylation of CTCF is a key regulatory mechanism for gene imprinting and ribosomal gene expression.
The interplay between CTCF, PARylation, and PARP highlights a significant regulatory network in genome methylation.