Related Experiment Videos
Plateletpheresis with the new COBE Spectra
V Kretschmer1, W Rossa, G Eisenhardt
1Department of Transfusion Medicine and Coagulation Physiology, University Clinics, Marburg, FRG.
Infusionstherapie (Basel, Switzerland)
|June 1, 1991
Summary
The COBE Spectra system efficiently collects platelets, achieving high separation efficiency with minimal cell contamination. Modifications to reduce anticoagulant citrate dextrose (ACD) slightly lowered yields but maintaining the ACD algorithm is crucial for platelet quality.
Area of Science:
- Hematology
- Medical Device Technology
Background:
- Plateletpheresis is a critical procedure for collecting platelets for transfusion.
- Optimizing collection efficiency and donor safety is an ongoing area of research.
- The COBE Spectra system is a device used for automated platelet collection.
Purpose of the Study:
- To evaluate the performance of the COBE Spectra system in plateletpheresis.
- To assess the impact of modified protocols on platelet yield, cell contamination, and anticoagulant usage.
- To determine optimal parameters for reducing anticoagulant citrate dextrose (ACD) consumption while maintaining platelet quality.
Main Methods:
- The COBE Spectra system was used in 71 plateletpheresis procedures.
- Standard collection and anticoagulant algorithms were employed for initial evaluations (n=57).
- Modifications included altering the ACD/blood ratio and increasing blood flow rate in subsequent runs (n=14).
Main Results:
- The system achieved a mean separation efficiency of 70.2% +/- 12.1%, collecting 4.3 x 10(11) +/- 1.2 x 10(11) platelets.
- Cell contamination (leukocytes and red cells) was minimal.
- Modifications with a 1:9 ACD/blood ratio and 50 ml/min blood flow resulted in significantly lower yields (3.1 x 10(11) +/- 0.7 x 10(11), p<0.01) and increased platelet aggregation.
Conclusions:
- The COBE Spectra system demonstrates effective platelet collection with high efficiency and low cell contamination.
- Reducing ACD/blood ratio significantly impacts yield and can lead to platelet activation.
- Maintaining the standard ACD algorithm is necessary to prevent platelet activation, though a reduction to approximately 15% ACD may be acceptable.