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Plateletpheresis with the new COBE Spectra
V Kretschmer1, W Rossa, G Eisenhardt
1Department of Transfusion Medicine and Coagulation Physiology, University Clinics, Marburg, FRG.
The COBE Spectra was evaluated in 71 plateletpheresis procedures. Using the collection and anticoagulant algorithms of the system (n = 57) we collected 4.3 +/- 1.2 x 10(11) platelets with a mean separation efficiency of 70.2 +/- 12.1%. The cell contamination was very low (leukocytes 0.5 +/- 1.0 x 10(7), red cells 1.5 +/- 2.2 x 10(7]. In four different modifications of the standard separation protocols, we tried to reduce the ACD consumption in order to shorten the donation time and to improve donor safety. A constant ACD/blood ratio of 1:9 and increase of the blood flow to 50 ml/min (n = 14) caused significantly lower yields (3.1 +/- 0.7, p less than 0.01) and visible spontaneous platelet aggregates in the collection line in 50% and in the PC's in 29% of the runs. In order to prevent platelet activation the ACD algorithm had to be maintained, but a reduction of the ACD/blood ratio to about 15% was acceptable.
The COBE Spectra was evaluated in 71 plateletpheresis procedures. Using the collection and anticoagulant algorithms of the system (n = 57) we collected 4.3 +/- 1.2 x 10(11) platelets with a mean separation efficiency of 70.2 +/- 12.1%. The cell contamination was very low (leukocytes 0.5 +/- 1.0 x 10(7), red cells 1.5 +/- 2.2 x 10(7]. In four different modifications of the standard separation protocols, we tried to reduce the ACD consumption in order to shorten the donation time and to improve donor safety. A constant ACD/blood ratio of 1:9 and increase of the blood flow to 50 ml/min (n = 14) caused significantly lower yields (3.1 +/- 0.7, p less than 0.01) and visible spontaneous platelet aggregates in the collection line in 50% and in the PC's in 29% of the runs. In order to prevent platelet activation the ACD algorithm had to be maintained, but a reduction of the ACD/blood ratio to about 15% was acceptable.