Isolation and identification of hemostatic ingredients from Lamiophlomis rotata (Benth.) Kudo

Lamiophlomis rotata (Benth.) Kudo (LR) is a traditional drug used by Chinese minorities such as Tibetans, Mongolians and Na Xi with the beneficial effects of hemostasis and alleviating pain. Flavonoids ingredients (P(1)), iridoid glycosides ingredients (P(2)) and maximus polarity components ingredients (P(3)) were isolated from the aqueous extract of LR (AELR) with polyamide and macroporous adsorptive resins chromatographic columns. The hemostatic activity of these ingredients was estimated by the changes in bleeding time (BT), clotting time (CT) and blood coagulation parameters in mice or rats. RP-HPLC was used to analyze the chemical composition of P(2). As a result, AELR and P(2) significantly shortened BT, CT and thrombin time (TT), and increased fibrinogen (FIB), but P(1) and P(3) showed no hemostatic effect during the experiment. Moreover, P(2) showed satisfactory hemostatic effect with dose-effect relationship and low toxicity in mice. 8-Dehydroxy shanzhiside, phloyoside II, shanzhiside methylester, loganin, and 8-O-acetylshanzhiside methylester were detected as the main iridoid glycosides in P(2) by RP-HPLC. Our results suggest that iridoid glycosides may be the hemostatic activity ingredients of L. rotata and are a potential hemostatic medicine.