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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

Genomic location analysis by ChIP-Seq.

Artem Barski1, Keji Zhao

  • 1Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA. barskia@nhlbi.nih.gov

Journal of Cellular Biochemistry
|January 29, 2009
PubMed
Summary
This summary is machine-generated.

Chromatin immunoprecipitation sequencing (ChIP-Seq) maps DNA-protein interactions genome-wide with high resolution. This review covers ChIP-Seq technology, analysis, and applications for understanding gene regulation networks.

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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
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Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • Gene expression and cell function are regulated by interactions between proteins and the genome.
  • Understanding these interactions is crucial for deciphering gene regulatory networks.

Purpose of the Study:

  • To review the ChIP-Seq technology for mapping DNA-protein interactions.
  • To discuss its applications, data analysis, and potential challenges.

Main Methods:

  • ChIP-Seq enables genome-wide mapping of DNA-protein interactions.
  • Provides high-resolution data on protein binding sites.

Main Results:

  • ChIP-Seq has revolutionized the study of DNA-protein interactions.
  • Facilitates a comprehensive understanding of gene regulation.

Conclusions:

  • ChIP-Seq is a powerful tool for identifying transcription factor binding targets.
  • Essential for unraveling complex gene regulatory networks and cell differentiation processes.