Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harnessing CRISPR-Cas12 and Microfluidics Chips for Multiplex Respiratory Pathogens Diagnosis.

ACS sensors·2026
Same author

Smartphone-Based Microbubble-Linked Immunosorbent Assay Powered by Classification-Regression Integrated Deep Learning for Portable Quantitative Biomarker Analysis.

ACS nano·2026
Same author

Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.

Analytical chemistry·2026
Same author

Gravity-Driven Formation of Water-in-Wax Spheres for Efficient One-Pot CRISPR Diagnostics.

ACS nano·2026
Same author

AI-integrated smartphone platform enables POC dual-channel glucose monitoring with an indicator-free nanozyme gel kit.

Biosensors & bioelectronics·2026
Same author

High-throughput identification of endogenous biomolecular condensates and phase-separating proteins.

Nature protocols·2026
Same journal

An Overview of Pterin Analysis in Biological Samples: From Occurrence and Properties to Sample Pretreatment Combined With Hyphenated Separation Techniques.

Journal of separation science·2026
Same journal

Chromatographic Purification of Complex Natural Products as a Decision Problem: Retention Prediction, Adaptive Optimization, and Experimental Feedback.

Journal of separation science·2026
Same journal

A High-Throughput Analytical Approach Using Polyaniline Doped With Oxalic Acid in Thin Film Solid-Phase Microextraction for the Determination of Personal Care Products in Recreational Waters.

Journal of separation science·2026
Same journal

Simultaneous Determination of Dechlorane-Related Compounds in Fish Muscle by Microwave-Assisted Extraction Combined With Enhanced Matrix Removal for Lipids Cleanup and Gas Chromatography-Tandem Mass Spectrometry.

Journal of separation science·2026
Same journal

Covalent Zwitterionic Peptide-Based Antifouling Coating of the Fused Silica Capillary Applied for CE Separation of Proteins.

Journal of separation science·2026
Same journal

Pharmacokinetic Study of Five Lead Components of Psoraleae Fructus in Human Serum by UHPLC-Q-TOF-MS and UHPLC-QqQ-MS/MS after Oral Administration of Qing'e Pills.

Journal of separation science·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
06:14

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Published on: September 26, 2025

Microseparation of membrane proteins.

Zhaowei Zhang1, Xiaojun Feng, Wei Du

  • 1Division of Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Journal of Separation Science
|January 29, 2009
PubMed
Summary
This summary is machine-generated.

Investigating low-abundance membrane proteins is challenging. Microseparation techniques like capillary electrophoresis (CE), micro-LC, and microchip methods offer high throughput and low sample consumption for efficient membrane protein analysis.

More Related Videos

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
08:24

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Published on: August 18, 2017

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
10:53

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo

Published on: November 7, 2013

Related Experiment Videos

Last Updated: Jun 26, 2026

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
06:14

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Published on: September 26, 2025

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
08:24

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Published on: August 18, 2017

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
10:53

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo

Published on: November 7, 2013

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Membrane proteins are crucial for cellular functions but are difficult to study due to low natural abundance and poor solubility.
  • Efficient separation of these proteins is a significant hurdle in biological and biochemical research.

Purpose of the Study:

  • To review recent advancements in microseparation techniques for membrane protein analysis.
  • To highlight the advantages of microseparation in overcoming challenges associated with membrane protein investigation.

Main Methods:

  • Discussion of key microseparation methods: Capillary Electrophoresis (CE), micro-Liquid Chromatography (micro-LC), and microchip-based separations.
  • Overview of sample preparation techniques including solubilization and preconcentration of membrane proteins.

Main Results:

  • Microseparation approaches offer high throughput, reduced analysis time, and minimal sample requirements.
  • These techniques are effective in addressing the challenges of separating low-abundance and insoluble membrane proteins.

Conclusions:

  • Microseparation technologies are vital tools for advancing membrane protein research.
  • Future applications of microseparation in membrane protein studies are anticipated to expand significantly.