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Published on: February 28, 2017
[Effect of Jagged-1 on the cytokine expression of murine myeloid-derived dendritic cells]
Zhi-yuan Fang1, Fei-yue Xing, Zhong-feng Guo
1Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, China.
Aim:
To investigate the in vitro effect of soluble Jagged-1/Fc on the cytokine expression of murine myeloid-derived dendritic cells induced by both rmGM-CSF and rmIL-4.
Methods:
The DC culture model under rmGM-CSF plus rmIL-4 conditions was established. The influences of Jagged-1/Fc on the morphology of DC was checked, and the cytokine expression were analyzed by Luminex system and ELISA. The activation capacity of Jagged-1/Fc-induced DC for allogeneic lymphocytes was detected by MTT assay.
Results:
Compared with LPS or Zymosan A, the amount of IL-4 expression of Jagged-1/Fc-induced DC increased significantly and the amount of TNF-alpha expression decreased markedly but gamma-secretase inhibitor DAPT reversed the inhibition of Jagged-1/Fc on TNF-alpha expression from DC and returned it to the control level. Jagged-1/Fc altered the amount of IL-10, IL-6, IL-2, IL-12 and IFN-gamma expression unconspicuously and had no effect on TGF-beta expression. The mixed lymphocyte reaction(MLR) revealed that Jagged-1/Fc-induced DC had the weakest ability to activate allogeneic lymphocytes and LPS-induced DC showed the strongest activation ability.
Conclusion:
The results indicate that Jagged-1/Fc-induced DC incline to induce immune tolerance and to prime naïve T cells to the Th2 cells.
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