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Updated: Jun 26, 2026

Whole Vitreous Humor Dissection for Vitreodynamic Analysis
Published on: May 24, 2015
Visualising vitreous through modified trans-scleral illumination by maximising the Tyndall effect
1Rotterdam Eye Hospital, Schiedamsevest 180, Rotterdam, The Netherlands. m.veckeneer@oogziekenhuis.nl
Background:
A new technique for visualisation of the vitreous base is described. It uses a standard lightpipe for scleral indentation and transillumination. Visualisation of the vitreous using low light levels can be achieved by enhancing the Tyndall effect.
Discussion:
Perfluorocarbon liquid (PFCL) is used to confine the aqueous environment to the anterior vitreous cavity and triamcinolone is added to increase light scatter. The technique clearly differentiates vitreous from PFCL and infusion fluid, and facilitates trimming of the vitreous base, draining of subretinal fluid and air/fluid exchange.
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