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Updated: Jun 26, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Solid-phase synthesis of oligonucleotide conjugates useful for delivery and targeting of potential nucleic acid

Harri Lönnberg1

  • 1Department of Chemistry, University of Turku, FIN-20014 Turku, Finland. harlon@utu.fi

Bioconjugate Chemistry
|January 30, 2009
PubMed

Insights

Oligonucleotide drugs face challenges in cellular delivery. This review covers advances in solid-supported synthesis for creating effective oligonucleotide conjugates to improve drug delivery and targeting.

Area of Science:

  • Medicinal Chemistry
  • Drug Delivery
  • Biotechnology

Background:

  • Oligonucleotide-based drugs show potential as a new chemotherapy approach.
  • Key challenges include inefficient cellular uptake and endosomal escape for nucleic acid therapeutics.
  • Covalent conjugation of oligonucleotides can enhance cellular internalization, targeting, and pharmacokinetics.

Purpose of the Study:

  • To review advancements in the solid-supported synthesis of oligonucleotide conjugates.
  • To highlight methods for overcoming delivery and targeting challenges in nucleic acid therapeutics.
  • To emphasize the importance of efficient chemistry for developing oligonucleotide drug conjugates.

Main Methods:

  • Focus on solid-supported synthesis techniques for oligonucleotide conjugation.
  • Discussion of chemical strategies for creating various types of oligonucleotide conjugates.
  • Review of methods facilitating cellular delivery and targeting of nucleic acid drugs.

Main Results:

  • Summarizes progress in solid-supported synthesis of oligonucleotide conjugates.
  • Highlights the role of conjugation in improving cellular uptake and endosomal escape.
  • Demonstrates the importance of conjugation for targeted delivery of nucleic acid therapeutics.

Conclusions:

  • Solid-supported synthesis is crucial for developing effective oligonucleotide conjugates.
  • Conjugation strategies are vital for overcoming major hurdles in nucleic acid drug delivery.
  • Advancements in synthesis pave the way for applicable nucleic acid therapeutics.

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