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Published on: September 7, 2018
Cryopreservation of canine platelets
E H Appleman1, B S Sachais, R Patel
1Schools of Veterinary Medicine and Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Journal of Veterinary Internal Medicine
|January 30, 2009
Summary
Cryopreservation of canine platelets using Thrombosol did not improve function or survival compared to 6% DMSO. However, cryopreserved platelets remain viable for potential therapeutic use when fresh platelets are unavailable.
Area of Science:
- Veterinary Medicine
- Hematology
- Transfusion Medicine
Background:
- Platelet cryopreservation enables long-term storage and immediate availability of transfusion products.
- Optimizing cryopreservation methods is crucial for maintaining platelet function and viability.
Purpose of the Study:
- To evaluate if Thrombosol (2% DMSO) enhances in vitro function and in vivo survival of cryopreserved canine platelets compared to 6% DMSO.
- To determine the efficacy of Thrombosol as a cryoprotective agent for canine platelets.
Main Methods:
- A prospective study involving thirty-three research dogs.
- Canine apheresis platelet concentrates (PCs) were cryopreserved in 6% DMSO or Thrombosol (2% DMSO).
- In vitro function and in vivo survival (using biotinylated platelets) were assessed 1-10 weeks post-freezing.
Main Results:
- Platelet aggregation was reduced in cryopreserved PCs compared to fresh PCs.
- Cryopreserved platelets showed lower P-selectin expression upon activation than fresh platelets.
- In vivo recovery and half-life of cryopreserved platelets were significantly lower than fresh platelets, with no significant difference between 6% DMSO and Thrombosol groups.
Conclusions:
- Thrombosol did not offer an advantage over 6% DMSO for canine platelet cryopreservation.
- Cryopreserved platelets retain the ability to be activated in vitro and can be therapeutically beneficial.
- Further research is required to evaluate the in vivo hemostatic function of cryopreserved platelets.
