Related Experiment Video
Updated: Jun 26, 2026
10:46
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Canine duplication of Descemet's membrane
C Kafarnik1, C J Murphy, R R Dubielzig
1Department of Pathobiological Sciences, Comparative Ocular Research Laboratories of Wisconsin, School of Veterinary Medicine, UW-Madison, Madison, WI, USA.
Veterinary Pathology
|January 30, 2009
Summary
This study describes a duplication of the canine Descemet
Area of Science:
- Veterinary Ophthalmology
- Ocular Pathology
- Canine Eye Diseases
Background:
- Descemet's membrane (DM) duplication is a rare condition in canine eyes.
- Understanding its morphology and association with ocular disease is crucial for diagnosis and treatment.
Purpose of the Study:
- To describe the morphology of canine Descemet's membrane duplication.
- To correlate the phenomenon with signalment, ocular history, and disease status.
Main Methods:
- Histopathological examination of 66 canine eyes using various stains (H&E, PAS, Masson's trichrome, picrosirius red).
- Immunohistochemistry for cytokeratin (CK), vimentin, and alpha-smooth muscle actin (SMA).
- Transmission electron microscopy (TEM) on select cases.
Main Results:
- DM duplication confirmed as basement membrane material by PAS and TEM.
- No significant difference in thickness between the original and duplicated DM.
- A fibrous, collagenous matrix with vimentin-positive spindle cells present in 39% of eyes.
- Corneal endothelial cells showed variable CK staining.
Conclusions:
- Canine DM duplication involves basement membrane material and can include a collagenous matrix with spindle cells.
- Associated ocular conditions include chronic glaucoma, intraocular surgery, lens luxation, and trauma.
- Endothelial cell activation and migration may play a role in pathogenesis.
More Related Videos
Related Concept Videos
01:05Desmosomes
The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein complexes comprising desmosomal...
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...