Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Folding01:22

Protein Folding

Overview
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding01:22

Protein Folding

Overview
Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
Protein Organization01:13

Protein Organization

Overview
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Eco-friendly synthesis and evaluation of carbon dots from kitchen waste for potential antibacterial applications.

Scientific reports·2026
Same author

Corrigendum to 'Biosynthesis, characterisation and biocompatibility of a unique and elastomeric medium chain-length polyhydroxyalkanoate for kidney glomerular tissue engineering' [Mater. Today Bio 33 (2025), 101932].

Materials today. Bio·2026
Same author

Heat Shock Proteins as Cancer Biomarkers: From Mechanism to Clinical Application.

Molecular diagnosis & therapy·2026
Same author

Modulation of biomolecular condensation of alpha-synuclein variants by eprodisate.

Communications chemistry·2026
Same author

Therapeutic aptamers in drug discovery: future elements of the pharmaceutical arsenal?

Expert opinion on drug discovery·2026
Same author

Optimisation of electrospinning parameters to successfully obtain high ratios of medium chain length polyhydroxyalkanoate in electrospun fibres with drug loading for wound healing applications.

Journal of materials science. Materials in medicine·2026

Related Experiment Video

Updated: Jun 26, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
09:27

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

Effect of trehalose on protein structure.

Nishant Kumar Jain1, Ipsita Roy

  • 1Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Punjab 160062, India.

Protein Science : a Publication of the Protein Society
|January 30, 2009
PubMed
Summary

Trehalose, a non-mammalian sugar, protects cells from environmental stress by preventing protein denaturation. Its protective roles extend to cryopreservation and organ preservation, revealing new therapeutic applications.

More Related Videos

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
08:37

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

Published on: March 21, 2025

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

Related Experiment Videos

Last Updated: Jun 26, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
09:27

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
08:37

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

Published on: March 21, 2025

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Trehalose, a disaccharide found in various organisms but not mammals, was historically considered a glucose storage molecule.
  • Recent research has redefined trehalose's role, highlighting its function as a stress-responsive factor crucial for cellular integrity under adverse conditions.

Purpose of the Study:

  • To review the evolving understanding of trehalose's functions over the past decade.
  • To propose unifying mechanisms by which trehalose stabilizes protein structures against denaturation and aggregation.

Main Methods:

  • Literature review focusing on studies published within the last 10 years.
  • Analysis of trehalose's molecular structure and its interactions with proteins and cellular environments.
  • Examination of trehalose's protective effects in various stress models and preservation applications.

Main Results:

  • Trehalose synthesis is induced by environmental stresses (heat, cold, desiccation, oxidation), aiding cellular adaptation.
  • Trehalose prevents protein denaturation and aggregation, including polyglutamine-mediated aggregation, by stabilizing protein structures.
  • Trehalose demonstrates efficacy in cryopreservation of sperm and stem cells and in organ preservation solutions.

Conclusions:

  • Trehalose's functions extend beyond energy storage to critical roles in cellular protection and preservation.
  • The stabilizing effect of trehalose on protein structure is a key mechanism underlying its diverse protective capabilities.
  • Understanding trehalose's mechanisms offers potential for novel therapeutic strategies in stress-related diseases and regenerative medicine.