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Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans
L Debussche1, M Couder, D Thibaut
1Département Analyse, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.
Journal of Bacteriology
|October 1, 1991
Abstract:
Cob(I)alamin adenosyltransferase (EC 2.5.1.17) was purified to homogeneity from extracts of a Pseudomonas denitrificans recombinant strain and sequenced at its N terminus. It is a homodimer (each unit with an Mr of 28,000) encoded by cobO. The enzyme adenosylated all of the corrinoids isolated from this microorganism but did not adenosylate cobyrinic acid.