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Variations of Candida albicans electrophoretic karyotypes
1Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642.
Journal of Bacteriology
|October 1, 1991
Summary
Spontaneous mutations in Candida albicans lead to chromosomal changes, generating genetic diversity. These karyotype alterations in morphological mutants and clinical isolates mirror natural variation in this species.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Candida albicans exhibits spontaneous morphological mutations linked to chromosomal aberrations.
- Previous studies identified 14 such mutants, but further investigation with improved techniques was needed.
Purpose of the Study:
- To investigate karyotype variations in clinical isolates and additional morphological mutants of Candida albicans.
- To understand the genetic basis of phenotypic diversity in Candida albicans.
Main Methods:
- Utilized improved chromosome separation techniques and hybridization probes.
- Analyzed karyotypes of 23 new morphological mutants and 4 clinical isolates.
- Examined progeny from unstable mutants to determine rates of instability.
Main Results:
- All 23 new morphological mutants displayed altered karyotypes, similar to previously identified ones.
- Chromosome VIII, carrying ribosomal DNA, showed frequent alterations.
- Unstable mutants continuously generated diverse karyotypes and phenotypes.
- Clinical isolates exhibited distinct electrophoretic karyotypes and morphologies, resembling spontaneous mutants.
Conclusions:
- Chromosomal alterations are a significant source of genetic variability in Candida albicans.
- Spontaneous chromosomal changes observed in laboratory conditions likely reflect natural genetic diversification mechanisms.
- Findings support the hypothesis that karyotypic variability contributes to the adaptability of Candida albicans in nature.