Related Experiment Video

Updated: Jun 26, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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Regulation by c-Myc of ncRNA expression

Niall S Kenneth1, Robert J White

  • 1Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK.

Current Opinion in Genetics & Development
|January 31, 2009
PubMed

Insights

The proto-oncogene c-Myc, a transcription factor, drives cancer by regulating protein-encoding genes and non-coding RNAs (ncRNAs). Understanding c-Myc

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Deregulated c-Myc activity is a key driver in numerous human cancers.
  • Research historically focused on c-Myc's regulation of protein-encoding genes.

Purpose of the Study:

  • To investigate the role of c-Myc in regulating non-coding RNA (ncRNA) production.
  • To understand the contribution of ncRNAs to c-Myc-associated cancer pathology.

Main Methods:

  • Analysis of c-Myc's transcriptional regulation.
  • Identification of regulated ncRNAs (tRNA, rRNA, miRNAs).
  • Assessment of RNA polymerase activity under c-Myc control.

Main Results:

  • c-Myc regulates the production of various non-coding RNAs, including tRNA, rRNA, and miRNAs.
  • This regulation involves c-Myc's control over the transcriptional activity of three distinct RNA polymerases.
  • ncRNAs play a significant role in the complex biology and pathology of c-Myc-driven cancers.

Conclusions:

  • c-Myc's role in cancer extends beyond protein-encoding genes to include significant regulation of ncRNAs.
  • These ncRNAs are crucial mediators of c-Myc's oncogenic functions.
  • Targeting c-Myc-regulated ncRNAs may offer new therapeutic strategies for cancer.

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