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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Small interfering RNAs (siRNA)

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: Jun 26, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

Knocking down barriers: advances in siRNA delivery.

Kathryn A Whitehead¹, Robert Langer, Daniel G Anderson

¹Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.

Nature Reviews. Drug Discovery

|January 31, 2009

Summary

RNA interference (RNAi) therapeutics show promise for treating diseases, but effective drug delivery remains a challenge. Novel synthetic materials are being developed for safe and efficient nucleic acid delivery.

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Last Updated: Jun 26, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Area of Science:

Biotechnology
Molecular Biology
Drug Delivery Systems

Background:

RNA interference (RNAi) has been a Nobel prize-winning discovery with significant investment in human therapeutics.
Clinical trials for RNAi therapies targeting age-related macular degeneration and respiratory syncytial virus show promising results.
Widespread application of RNAi therapeutics necessitates the development of safe and effective drug delivery vehicles.

Purpose of the Study:

To provide an update on the progress of RNAi therapeutics.
To highlight novel synthetic materials for nucleic acid encapsulation and intracellular delivery.

Main Methods:

Review of current progress in RNAi therapeutics.
Identification and discussion of novel synthetic materials for drug delivery.

Main Results:

Early clinical successes indicate the potential of RNAi therapeutics.
Novel synthetic materials offer potential solutions for safe and effective nucleic acid delivery.
Progress in encapsulation and intracellular delivery techniques is crucial for therapeutic advancement.

Conclusions:

Advancements in synthetic materials are critical for the clinical success of RNAi therapeutics.
Continued research into drug delivery vehicles will accelerate the use of gene silencing for disease treatment and prevention.