Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...
Antimicrobial Proteins01:23

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Inflammation01:38

Inflammation

Overview
Acute Inflammation III: Local and Systemic Effects01:25

Acute Inflammation III: Local and Systemic Effects

Acute inflammation produces a coordinated set of local and systemic changes that limit injury, eliminate pathogens, and initiate repair. These responses arise within minutes of infection, trauma, or chemical insult and are driven by vascular alterations and leukocyte-derived mediators. When the stimulus resolves, the reaction typically abates within days.Local EffectsAt the site of injury, arteriolar vasodilation increases blood flow, resulting in redness and warmth. Simultaneously, increased...
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced community first responder support system: protocol of a randomized trial to improve bystander cardiopulmonary resuscitation quality for out-of-hospital cardiac arrest patients.

Resuscitation plus·2025
Same author

Corrigendum to "The "invisible ceiling" of bystander CPR in three Asian countries: Descriptive study of national OHCA registry" [Resuscitation 206 (2025) 110445].

Resuscitation·2025
Same author

Cost-effectiveness analysis of a 'Termination of Resuscitation' protocol for the management of out-of-hospital cardiac arrest.

Resuscitation·2024
Same author

Machine learning prediction of refractory ventricular fibrillation in out-of-hospital cardiac arrest using features available to EMS.

Resuscitation plus·2024
Same author

Comparing outcomes of out-of-hospital cardiac arrest patients with initial shockable rhythm in Singapore and Osaka using population-based databases.

Critical care (London, England)·2023
Same author

The Association of <i>Helicobacter pylori</i> Biofilm with Enterovirus 71 Prolongs Viral Viability and Survival.

International journal of molecular sciences·2023

Related Experiment Video

Updated: Jun 26, 2026

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation
07:48

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Published on: May 16, 2016

Local inflammation induces complement crosstalk which amplifies the antimicrobial response.

Jing Zhang1, Jingyun Koh, Jinhua Lu

  • 1NUS Graduate School for Integrative Science and Engineering, National University of Singapore, Singapore.

Plos Pathogens
|January 31, 2009
PubMed
Summary

Local inflammation conditions trigger a potent immune response against Pseudomonas aeruginosa. This involves a novel interaction between C-reactive protein (CRP) and L-ficolin, enhancing complement pathways to kill bacteria.

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Related Experiment Videos

Last Updated: Jun 26, 2026

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation
07:48

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Published on: May 16, 2016

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Area of Science:

  • Immunology
  • Microbiology
  • Biochemistry

Background:

  • The human immune system combats pathogens via inflammation, increasing acute phase proteins like C-reactive protein (CRP) and cytokines.
  • Pseudomonas aeruginosa is an opportunistic pathogen, often antibiotic-resistant, posing significant challenges in inflamed tissues.
  • Understanding host immune responses to P. aeruginosa in inflammatory conditions is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the mechanism of P. aeruginosa killing in plasma under local infection-inflammation conditions.
  • To elucidate the role of plasma protein interactions in enhancing antimicrobial activity.
  • To explore the contribution of complement pathways to host defense against P. aeruginosa.

Main Methods:

  • Analysis of blood samples from P. aeruginosa-infected patients under simulated local inflammation conditions (acidosis, low calcium).
  • Measurement of C-reactive protein (CRP) and L-ficolin interaction.
  • Assays for complement component C4 deposition, phagocytosis, and protein competition.

Main Results:

  • P. aeruginosa was effectively killed in plasma under simulated local inflammation (pH 6.5, 2 mM calcium).
  • A 100-fold increase in CRP-L-ficolin interaction was observed, sensitive to pH and calcium levels.
  • This interaction triggered autonomous complement amplification pathways, reinforcing classical and lectin pathways.

Conclusions:

  • Local infection-inflammation conditions induce a pH- and calcium-dependent CRP-L-ficolin interaction.
  • This interaction activates potent, autonomous complement amplification pathways enhancing bacterial killing.
  • Findings offer insights into host defense against P. aeruginosa and potential therapeutic strategies.