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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Oxidized high-density lipoprotein promotes maturation and migration of bone marrow derived dendritic cells from
Zeng-xiang Xu1, Yong-zong Yang, Da-ming Feng
1Department of Pathology, Wannan Medical College, Wuhu, Anhui 241000.
Insights
Oxidized high-density lipoprotein (oxHDL) enhances dendritic cell maturation and migration in vitro. This study shows oxHDL promotes key immune cell functions, suggesting a role in immune responses.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Dendritic cells (DCs) are crucial antigen-presenting cells in the immune system.
- Oxidized high-density lipoprotein (oxHDL) is implicated in various biological processes.
- The specific effects of oxHDL on bone marrow-derived dendritic cell (BMDC) function require further elucidation.
Purpose of the Study:
- To investigate the impact of oxHDL on the maturation and migration of BMDCs.
- To compare the effects of oxHDL with controls, including native HDL and lipopolysaccharide (LPS).
Main Methods:
- BMDCs were isolated from C57BL/6J mice and cultured with recombinant cytokines.
- Cells were stimulated with oxHDL (50 μg/mL), HDL (50 μg/mL), PBS, or LPS (1 μg/mL).
- Maturation markers (CD86, MHCII), T cell proliferation (MLRs), cytokine secretion (IL-12, IL-10), and cell migration were assessed.
Main Results:
- oxHDL significantly increased CD86 and MHCII expression, T cell proliferation, IL-12 and IL-10 secretion, and cell migration compared to PBS.
- LPS also significantly increased these parameters, with a greater effect than oxHDL.
- oxHDL treatment resulted in approximately double the number of migrated cells compared to the HDL control group.
Conclusions:
- In vitro, oxHDL promotes the maturation of BMDCs, indicated by increased expression of maturation markers.
- oxHDL enhances the migratory capacity of BMDCs.
- These findings suggest oxHDL plays a role in modulating dendritic cell function and potentially influencing immune responses.
Objective:
To explore the influence of oxidized high-density lipoprotein (oxHDL) on the maturation and migration of bone marrow-derived dendritic cells (BMDCs) from C57BL/6J mice.
Methods:
The C57BL/6J mice bone marrow cell suspension was prepared and purified. Recombinant granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant interleukin-4 (rmIL-4) were used to promote monocytes to differentiate and suppress lymphocytes. Then 50 microg/mL oxHDL was added to stimulate BMDCs, using 50 microg/mL high-density lipoprotein (HDL) as homologous protein control, PBS as negative control, and 1 microg/mL lipopolysaccharide (LPS) as positive control. The CD86 and MHCII expression rates were detected with fluorescence-activated cell sorting (FACS). Liquid scintillation counting (LSC) was used in mixed lymphocyte reactions (MLRs) to reflect the ability of BMDCs in stimulating the proliferation of homologous T cells. Levels of cytokines IL-12 and IL-10 were detected by ELISA. The cell migration was evaluated with the transwell system.
Results:
Compared with PBS group, the expressions of CD86 and MHCII, counts per minute of MLRs, secretion of IL-12 and IL-10, and number of migrated cells in oxHDL group and LPS group significantly increased (all P<0.05), while the increment was less in oxHDL group than LPS group. The number of migrated cells in oxHDL group was about twice of that in HDL group.
Conclusion:
OxHDL may promote the maturation and migration of BMDCs in vitro.
