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Related Experiment Video

Updated: Jun 26, 2026

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
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Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

Published on: February 5, 2018

Banking North American buffalo semen.

C Lessard1, J Danielson, K Rajapaksha

  • 1Agriculture and Agri-Food Canada, Western College of Veterinary Medicine, Saskatoon, SK, Canada S7N 5B4. lessardc@agr.gc.ca

Theriogenology
|February 3, 2009
PubMed
Summary
This summary is machine-generated.

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14 Pregnancies produced after fixed-time artificial insemination using sex-sorted sperm in wood bison.

Reproduction, fertility, and development·2022

This study developed a method for collecting and cryopreserving bison semen. Wood bison semen showed higher motility in late summer, and a specific medium improved post-thaw sperm viability.

Area of Science:

  • Veterinary Reproduction
  • Conservation Genetics
  • Bison Biology

Background:

  • Effective semen collection and cryopreservation are crucial for managing bison populations.
  • Understanding seasonal variations in semen quality is important for reproductive success.

Purpose of the Study:

  • To establish a reliable procedure for collecting and cryopreserving semen from wood bison (Bison bison athabascae) and plains bison (Bison bison bison).
  • To evaluate the efficacy of different cryopreservation media and sperm sources.

Main Methods:

  • Semen was collected via electroejaculation from wood and plains bison bulls between June and October.
  • Sperm was also obtained from the cauda epididymis of plains bison.
  • Cryopreservation was performed using Triladyl (egg yolk-based) and Andromed (animal-product-free) media.

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  • Sperm morphology and motility were assessed for fresh and post-thawed samples.

    • Main Results:

    • Total sperm motility varied seasonally, with wood bison exhibiting higher motility in August and September compared to plains bison.
    • Post-thawed semen quality did not differ significantly between wood and plains bison.
    • Triladyl resulted in significantly higher post-thaw sperm motility than Andromed.
    • Post-thawed epididymal sperm showed higher motility than electroejaculated sperm when cryopreserved with Andromed.

    Conclusions:

    • Electroejaculation is an effective method for collecting high-quality bison semen.
    • Cryopreservation protocols can be optimized using specific media like Triladyl for improved post-thaw viability.
    • These findings contribute to the development of assisted reproductive technologies for bison conservation.