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An Assay for Quantifying Protein-RNA Binding in Bacteria
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An Assay for Quantifying Protein-RNA Binding in Bacteria

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DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes

Matej Butala1, Stephen J W Busby, David J Lee

  • 1School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.

Nucleic Acids Research
|February 3, 2009
PubMed
Summary

This study introduces DNA sampling, a rapid method to isolate specific DNA segments and bound proteins from Escherichia coli. The technique uses yeast I-SceI meganuclease and FLAG-tagged LacI repressor for efficient purification.

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