Related Experiment Video
Updated: Jun 26, 2026
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Combined hyperspectral and spectral domain optical coherence tomography microscope for noninvasive hemodynamic
Melissa C Skala1, Andrew Fontanella, Hansford Hendargo
1Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. melissa.skala@duke.edu
Abstract:
We have combined hyperspectral imaging with spectral domain optical coherence tomography (SDOCT) to noninvasively image changes in hemoglobin saturation, blood flow, microvessel morphology, and sheer rate on the vessel wall with tumor growth. Changes in these hemodynamic variables were measured over 24 h in dorsal skin fold window chamber tumors. There was a strong correlation between volumetric flow and hemoglobin saturation (rho=0.89, p=9x10(-6), N=15) and a moderate correlation between shear rate on the vessel wall and hemoglobin saturation (rho=0.56, p=0.03, N=15).
