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Related Experiment Video

Updated: Jun 26, 2026

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

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Published on: December 1, 2021

Fast-fluorescence dynamics in nonratiometric calcium indicators.

M Gersbach¹, D L Boiko, C Niclass

¹Ecole Polytechnique Fédérale de Lausanne, Quantum Architecture Group, Lausanne, Switzerland.

|February 3, 2009

Summary

High-affinity calcium indicator Oregon Green BAPTA-1 (OGB-1) fluorescence decay was analyzed with high temporal resolution. A triple exponential decay model accurately measures free Ca2+ concentration and dissociation constants for nonratiometric indicators.

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Last Updated: Jun 26, 2026

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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

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Published on: August 4, 2015

Area of Science:

Biochemistry
Analytical Chemistry
Optical Physics

Background:

Nonratiometric calcium indicators are crucial for monitoring intracellular calcium dynamics.
Oregon Green BAPTA-1 (OGB-1) is a high-affinity indicator widely used in biological research.
Accurate measurements of free Ca2+ concentration are essential for understanding cellular signaling.

Purpose of the Study:

To analyze the fluorescence decay kinetics of OGB-1 with high temporal resolution.
To develop a model for accurate quantification of free Ca2+ using nonratiometric indicators.
To determine the dissociation constants of OGB-1 for improved calcium measurements.

Main Methods:

Utilizing two-photon excitation microscopy for high spatial and temporal resolution.
Analyzing fluorescence decay curves of OGB-1.
Applying a triple exponential decay model to fluorescence data.

Main Results:

The fluorescence decay of OGB-1 is best described by a triple exponential function.
The study provides a validated model for precise free Ca2+ concentration measurements.
Dissociation constants for OGB-1 were determined, enhancing indicator characterization.

Conclusions:

The developed model enables accurate assessment of free Ca2+ using OGB-1.
Understanding OGB-1 fluorescence dynamics improves the reliability of calcium imaging studies.
This work advances the quantitative analysis of nonratiometric calcium indicators.