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Published on: December 1, 2021
Fast-fluorescence dynamics in nonratiometric calcium indicators
M Gersbach1, D L Boiko, C Niclass
1Ecole Polytechnique Fédérale de Lausanne, Quantum Architecture Group, Lausanne, Switzerland.
Abstract:
The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.
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