Evaluation of the DG29 test for early detection of pregnancy in cattle

The objective of this study was to evaluate a commercial blood test (DG29) for pregnancy in cattle. Compared with ultrasound, the sensitivity of the assay for detecting pregnancy around day 30 post-insemination was 99.4% [95% confidence interval (CI): 96.1-100]. The specificity was 100% (95% CI: 84.7-100) in non-inseminated cattle, and 66.7%; (95% CI: 49.7-80.4) in inseminated nonpregnant cattle. Positive and negative predictive values were 92.6% and 96.3%, respectively, in a sample of inseminated cattle in which 80% were pregnant.