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Differentiation of psittacine herpesvirus field isolates by restriction endonuclease analysis

A S Schroder-Gravendyck, E F Kaleta, R E Marschang

    Avian Pathology : Journal of the W.V.P.A
    |February 3, 2009
    PubMed

    Insights

    Psittacine herpesviruses (PsHV) cause Pacheco's parrot disease (PPD). DNA analysis revealed 12 distinct PsHV strains, confirming genetic diversity and identifying reinfection in parrots with a different PsHV strain.

    Area of Science:

    • Veterinary Virology
    • Avian Pathology
    • Molecular Epidemiology

    Background:

    • Psittacine herpesviruses (PsHV) are significant pathogens in parrots, causing Pacheco's parrot disease (PPD).
    • Previous studies differentiated PsHV strains based on serological relationships.

    Purpose of the Study:

    • To molecularly characterize PsHV field isolates using DNA restriction endonuclease analysis.
    • To compare genetic profiles with existing serological classifications.
    • To investigate the potential for reinfection with distinct PsHV strains in an aviary setting.

    Main Methods:

    • Isolation and identification of 31 PsHV field isolates from 15 psittacine species.
    • DNA analysis using restriction endonucleases (EcoRI, PstI, BglII).
    • Comparison of restriction endonuclease profiles with serological data.

    Main Results:

    • Identification of 12 distinct DNA restriction patterns among the 31 PsHV isolates.
    • Restriction profiles confirmed serological groupings but revealed finer genetic distinctions.
    • Demonstration of spontaneous reinfection with a genetically different PsHV strain in an aviary 4 years after an initial PPD outbreak.

    Conclusions:

    • Psittacine herpesviruses exhibit significant antigenic and genetic diversity.
    • Molecular typing provides a robust method for differentiating PsHV strains.
    • Understanding genetic diversity is crucial for managing PPD outbreaks and preventing reinfection.

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