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Updated: Jun 26, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Top-down proteomics with a quadrupole time-of-flight mass spectrometer and collision-induced dissociation
Andrea Armirotti1, Umberto Benatti, Gianluca Damonte
1Centro Biotecnologie Avanzate, Largo Rosanna Benzi 10, 16132 Genova, Italy. andrea.armirotti@unige.it
Abstract:
With slight modifications of the instrumental parameters, we demonstrate that satisfactory top-down data can be obtained with collision-induced dissociation (CID) tandem mass spectrometry on a quadrupole time-of-flight (qTOF) instrument not originally designed for this purpose. Protein identification is achieved with both N- and C-terminal sequence tags and BLAST database searches. The accurate mass measurement of multiply charged fragment ions (mostly y and b-type) supplements the limited set of cleavage sites and provides a high degree of sequence coverage (90-100%). Post-translational modification issues can be addressed too. This approach might help those mass spectrometry (MS) core facilities that are not able to afford very high-resolution instruments, thus expanding the benefits of top-down protein analysis over the worldwide MS community.
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