Langmuir-Blodgett film based on MEH-PPV for cholesterol biosensor

Cholesterol oxidase (ChOx) has been immobilized onto conducting poly[2-methoxy,5-(2'-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV)/stearic acid (SA) Langmuir-Blodgett film transferred onto octadecanethiol (ODT) modified gold plate. The ChOx/MEH-PPV/SA LB film bioelectrode exhibits has been characterized by FT-IR, contact angle, and atomic force microscopy. The response of the ChOx/MEH-PPV/SA LB film bioelectrode carried out using differential pulse voltammetry (DPV) studies reveal linearity from 1.29 to 12.91 mM of cholesterol concentration and response time as 30 s. This ChOx/MEH-PPV/SA bioelectrode exhibits values of correlation coefficient as 0.9939, standard deviation as 0.0029 microA and limit of detection as 1.66 mM. UV-visible spectrophotometer studies reveal that 5.2x10(-3) U of ChOx are actively working per cm(2) area of ChOx/MEH-PPV/SA LB film bioelectrode and this bioelectrode is thermally stable upto 55 degrees C with reusability of about 60 times.