Electron microscopy in yeast
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Updated: Jun 26, 2026

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins
Published on: April 26, 2024
1Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan.
Abstract:
The membrane dynamics of autophagy in yeast were resolved mostly by using various electron microscopy (EM) methods combined with the cryofixation technique. In addition, the similarity of the dynamic process between autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway was first clarified through EM studies. In this chapter the application of several EM methods to detect the dynamic events involving cellular structures are described, and examples are provided of the typical images obtained in autophagy and the Cvt pathway.
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