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Related Concept Videos

Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
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Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

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Related Experiment Video

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Monitoring Stub1-Mediated Pexophagy
08:26

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Published on: May 12, 2023

Pexophagy in Pichia pastoris.

Masahide Oku1, Yasuyoshi Sakai

  • 1CREST, Japan Science and Technology Agency, Japan, and Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

Methods in Enzymology
|February 3, 2009
PubMed
Summary

This study explores peroxisome turnover (pexophagy) in methylotrophic yeast. Researchers utilized live cell imaging and protein level analysis to understand pexophagy dynamics and identify key factors.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Yeast Genetics

Background:

  • Peroxisomes are vital organelles with tightly regulated quantities, responding to metabolic shifts.
  • Pexophagy, the autophagic degradation of peroxisomes, is crucial for cellular homeostasis.
  • Methylotrophic yeast, like Pichia pastoris, are model organisms for studying pexophagy due to their ability to utilize methanol.

Purpose of the Study:

  • To investigate the dynamics of peroxisome turnover (pexophagy) in the methylotrophic yeast Pichia pastoris.
  • To identify and characterize the molecular mechanisms governing pexophagy.
  • To establish robust methods for analyzing pexophagy at the molecular level.

Main Methods:

  • Live cell imaging to visualize dynamic membrane events during pexophagy.

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  • Quantification of peroxisomal proteins in cell lysates as an indicator of pexophagy completion.
  • Utilizing methylotrophic yeast strains capable of growth on methanol.

    • Main Results:

    • Demonstrated simultaneous visualization of multiple membrane dynamics during pexophagy in Pichia pastoris.
    • Established that decreased peroxisomal protein levels in cell lysates serve as a reliable marker for pexophagy completion.
    • Identified key factors and pathways involved in pexophagy through studies in methylotrophic yeast.

    Conclusions:

    • Pichia pastoris offers a powerful system for studying pexophagy dynamics in real-time.
    • The established methods provide a foundation for detailed molecular analyses of pexophagy.
    • Understanding pexophagy is critical for comprehending cellular adaptation to metabolic changes.