Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal
Related Experiment Video
Updated: Jun 26, 2026
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
Sung-Hee Cho1, Man Ho Choi, Won-Yong Lee
1Life Sciences Division, Korea Institute of Science and Technology, 39-1 Hawolkok-dong, Seoul, Korea.
Objective:
Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa).
Design And Methods:
Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n=150, age: 46.6+/-7.7 years), and female controls (n=150, age: 46.8+/-7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction.
Results:
Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001; 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N(2),N(2)-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients.
Conclusions:
This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.