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[Effects of mechanical stress on the formation and expression of Cbfalpha1 in vitro]
Xue-jin Wang1, Xiao-ning Hao, Wei Liu
1Dept. of Stomatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
Objective:
To evaluate the effects of mechanical stress on the formation and expression of core binding factor alpha1 (Cbfalpha1) in MG-63 cells cultured on titanium in vitro.
Methods:
MG-63 cells cultured on the titanium were subjected to a centrifugal force (2.205 N) 15 min per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfalpha1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction (RT-PCR).
Results:
Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force (P<0.05). Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfalpha1 and the relative mRNA level of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significant (P<0.05).
Conclusion:
Mechanical stress are beneficial to the formation and expression of Cbfalpha1.
