Related Experiment Video
Updated: Jun 26, 2026
08:26
Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
Hansenula polymorpha pex11 cells are affected in peroxisome retention
Arjen M Krikken1, Marten Veenhuis, Ida J van der Klei
1Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
The FEBS Journal
|February 4, 2009
Summary
The Hansenula polymorpha PEX11 gene regulates peroxisome proliferation. It is also crucial for peroxisome retention during cell division, a function previously unknown for PEX11.
Area of Science:
- Cell Biology
- Molecular Biology
- Yeast Genetics
Background:
- Peroxisome biogenesis and maintenance are critical cellular processes.
- Pex11p is known to regulate peroxisome proliferation in various organisms.
- The role of Pex11p in peroxisome segregation during cell division remains largely unexplored.
Purpose of the Study:
- To characterize the function of the Hansenula polymorpha PEX11 gene.
- To investigate the role of Pex11p in peroxisome proliferation and segregation.
- To explore the interplay between Pex11p and Inp1p in peroxisome dynamics.
Main Methods:
- Gene cloning and characterization of Hansenula polymorpha PEX11.
- Morphological analysis of peroxisomes in wild-type and mutant yeast strains.
- Time-lapse imaging of Inp1-green fluorescent protein localization during the cell cycle.
Main Results:
- Hansenula polymorpha PEX11 gene was successfully cloned and characterized.
- Pex11p modulates peroxisome proliferation, consistent with previous findings.
- pex11 cells exhibit defects in peroxisome retention in mother cells during division.
- H. polymorpha inp1 cells also show impaired peroxisome retention.
- Inp1-green fluorescent protein localization is cell-cycle dependent and normally recruited in pex11 cells.
Conclusions:
- H. polymorpha Pex11p is essential for both peroxisome proliferation and proper peroxisome segregation.
- Pex11p plays a dual role in peroxisome dynamics, impacting both organelle abundance and distribution.
- These findings reveal a novel function for Pex11p in ensuring accurate inheritance of peroxisomes during yeast cell division.
Related Concept Videos
01:27Protein Import into the Peroxisomes
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
01:24Peroxisomes
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
01:30Peroxisomes
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...
01:24Peroxisomes
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
01:22Lysosomal Hydrolases
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
01:33Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...