Related Experiment Video
Updated: Jun 26, 2026
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
Concordance between the AmpFlSTR MiniFiler and AmpFlSTR Identifiler PCR amplification kits in the Kuwaiti population
Mohammad A Alenizi1, William Goodwin, Sibte Hadi
1School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, UK. Mal-enizi@uclan.ac.uk
Abstract:
The AmpFlSTR MiniFiler polymerase chain reaction amplification kit, developed and supplied by Applied Biosystems, complements the AmpFlSTR Identifiler polymerase chain reaction amplification kit (Applied Biosystems, Warrington, U.K.) by improving the success rate when profiling DNA that is degraded or contains inhibitors. Before applying the MiniFiler kit to casework, the profiles from 200 unrelated Kuwaitis were compared to Identifiler profiles. Concordance was observed for 99.875% (1598 of 1600) of the compared STR loci. The two discordant profiles displayed allelic dropout: one at the D13S317 locus due to nonamplification of allele 10 in the MiniFiler profile, and one at the D18S51 locus due to nonamplification of allele 18 in the Identifiler profile.
