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Updated: Jun 26, 2026

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

Differential cell surface properties of vegetative Bacillus.

J E Thwaite¹, T R Laws, T P Atkins

¹Department of Biomedical Sciences, Defence Science and Technology Laboratory, Salisbury, Wiltshire, UK.

Letters in Applied Microbiology

|February 4, 2009

Summary

Bacterial cell hydrophobicity correlates with pathogenicity in Bacillus species. Highly hydrophobic Bacillus anthracis and Bacillus thuringiensis adhere more to mammalian cells than less hydrophobic Bacillus cereus and Bacillus subtilis.

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Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

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Last Updated: Jun 26, 2026

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

Area of Science:

Microbiology
Cell Biology
Biophysics

Background:

The genus Bacillus contains diverse species with varying pathogenic potential.
Understanding the physical-chemical properties of Bacillus is crucial for distinguishing pathogenic strains.

Purpose of the Study:

To determine the hydrophobicity of various Bacillus species.
To evaluate the correlation between bacterial hydrophobicity and pathogenicity.
To assess the adherence of Bacillus species to mammalian cell lines.

Main Methods:

Bacterial adhesion to hydrocarbon assays were employed to measure cell surface hydrophobicity.
Mammalian cell adhesion assays using A549 and J774 cell lines were performed.
Hydrophobicity profiles of vegetative Bacillus anthracis, Bacillus thuringiensis, Bacillus cereus, and Bacillus subtilis were compared.

Main Results:

Significant differences in hydrophobicity were observed among vegetative Bacillus species.
Bacillus anthracis and Bacillus thuringiensis exhibited high hydrophobicity.
Bacillus cereus and Bacillus subtilis displayed lower hydrophobicity.
A positive correlation was found between bacterial hydrophobicity and adherence to mammalian cell lines.

Conclusions:

Bacterial adherence to mammalian cells is linked to cell surface hydrophobicity.
Hydrophobicity correlates with the relative pathogenicity of certain Bacillus species.
Investigating physical-chemical properties can aid in rapid identification of pathogenic Bacilli.