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Published on: February 16, 2018
Mammalian cell-based biosensors for pathogens and toxins
Pratik Banerjee1, Arun K Bhunia
1Molecular Food Microbiology Laboratory, Center for Food Safety Engineering, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
Trends in Biotechnology
|February 4, 2009
Summary
Cell-based biosensors (CBBs) offer rapid detection of food, agriculture, and environmental threats. This review details advancements in mammalian CBBs using multidisciplinary approaches for improved hazard assessment.
Area of Science:
- Biotechnology
- Biosensor Technology
- Toxicology
Background:
- Cell-based biosensors (CBBs) are crucial for detecting food, agriculture, environmental, and biosecurity hazards.
- CBBs assess risks by detecting functional host-hazard interactions and physiological responses.
Purpose of the Study:
- To review progress in mammalian CBB development for pathogen and toxin detection.
- To emphasize multidisciplinary approaches integrating biology, physics, and engineering.
Main Methods:
- Review of literature on mammalian CBBs for hazard detection.
- Focus on integrating molecular biology and microbiology with physics and engineering.
- Development of 3D cell-culture systems and high-throughput screening (HTS) using optical and electrical methods.
Main Results:
- Mammalian CBBs show promise for rapid hazard identification.
- Multidisciplinary approaches have advanced CBB technology.
- 3D cell cultures and HTS systems enhance detection capabilities.
Conclusions:
- Cell-based biosensors are effective tools for risk assessment in various sectors.
- Interdisciplinary research is key to advancing CBBs for pathogen and toxin detection.
- Future developments will likely involve sophisticated cell culture and screening technologies.
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